Green microwave extraction of antioxidants and phenolic compounds from Moringa oleifera leaf: An optimization study using response surface methodology

Authors

  • Tze Kang Cheong Universiti Malaysia Pahang Al-Sultan Abdullah
  • Siti Kholijah Abdul Mudalip Universiti Malaysia Pahang Al-Sultan Abdullah
  • Nurmaryam Aini Hashim Universiti Malaysia Pahang Al-Sultan Abdullah
  • Rohaida Che Man Universiti Malaysia Pahang Al-Sultan Abdullah
  • Siti Zubaidah Sulaiman Universiti Malaysia Pahang Al-Sultan Abdullah
  • Shalyda Md. Shaarani Universiti Malaysia Pahang Al-Sultan Abdullah

DOI:

https://doi.org/10.15282/jceib.v10i2.11245

Keywords:

Green extraction, microwave irradiation, Moringa oleifera, Optimization, Antioxidant

Abstract

Moringa oleifera is a good source of nutrition and very beneficial for health improvement. This study aimed to optimize the process parameters for the extraction of bioactive substances from M. oleifera leaf using microwave-assisted extraction (MAE). The Response Surface Methodology (RSM) based on Box-Behnken designs (BBD) was used to optimise the process parameters including solid-to-solvent ratio (1 to 3 g/100 mL), microwave power (100 to 200 W), and extraction time (15 to 25 min) on the total phenol content (TPC) and antioxidant activity (AA).  The optimum conditions were 2.01 g/100 mL solid-to-solvent ratio, 152.7 W microwave power, and 20.54 min extraction time to obtained to obtain the optimum TPC and AA. The optimum TPC and DPPH were 529.801 mg GAE/g dry sample and 87.67%, respectively. The results were successfully fitted into the second-order polynomial model with R2 values of 0.9958 and 0.9865, respectively. The predicted TPC and AA deviate from the experimental data by 1.40% and 2.69%, respectively. Thus, the model accuracy is proved for the modelling of M. oleifera leaves extraction by MAE.

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Published

2024-12-30

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Articles