Utilization of Fermented Moringa and Trichanthera Gigantea Leaves as Protein Sources in Fish Meal Production

Authors

  • Azilah Ajit Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Gambang, Kuantan, Pahang, Malaysia
  • Nur Irdina Johari Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Gambang, Kuantan, Pahang, Malaysia
  • Ahmad Ziad Sulaiman Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Gambang, Kuantan, Pahang, Malaysia
  • Nurul Aini Mohd Azman Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Gambang, Kuantan, Pahang, Malaysia

DOI:

https://doi.org/10.15282/jceib.v11i2.11351

Keywords:

Fish waste, Moringa, Trichanthera gigantea, Fish pellet, Protein

Abstract

The increasing demand for sustainable protein sources in aquaculture drives innovation in fish meal production. This study investigates the use of fermented leaves, specifically Moringa leaves and Trichanthera gigantea leaves, as alternative protein sources. The objective is to identify the optimal ratio of these fermented leaves in fish meal to enhance protein concentration. Fish feed formulations with varying ratios undergo fermentation and drying, followed by analysis of their protein, lipid, moisture, and ash content. The protein content is standardized using a Bovine Serum Albumin (BSA) calibration curve and assessed pre-fermentation via the Lowry assay. Additionally, the floatability of the fish pellets is evaluated to determine their suitability in aquatic environments. Results indicate that a formulation containing 45 w/w% Moringa leaves, 45 w/w% rice bran, and 10 w/w% starch is the most promising, with a protein content of 28.94 %, lipid content of 10.37 %, moisture content of 10.08 %, and ash content of 7 %. The conclusion emphasizes that Moringa leaves serve as a superior protein source compared to Trichanthera gigantea leaves for fish meal production, thereby supporting sustainable aquaculture practices.

 

 

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Published

30-12-2025

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How to Cite

1.
Ajit A, Johari NI, Sulaiman AZ, Mohd Azman NA. Utilization of Fermented Moringa and Trichanthera Gigantea Leaves as Protein Sources in Fish Meal Production. J. Chem. Eng. Ind. Biotechnol. [Internet]. 2025 Dec. 30 [cited 2026 May 15];11(2):63-71. Available from: https://journal.ump.edu.my/index.php/jceib/article/view/11351

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