Performance analysis of immobilized and co-immobilized enriched-mixed culture for hydrogen production

Astrilia Damayanti; Sarto; Wahyudi B. Sediawan; Siti Syamsiah

doi:10.15282/jmes.12.1.2018.18.0312

Authors

Astrilia Damayanti Department of Chemical Engineering, Faculty of Engineering, Universitas Negeri Semarang, Kampus Sekaran, Gunungpati, Semarang, Indonesia
Sarto Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada, Jalan Grafika No. 2, Yogyakarta, Indonesia
Wahyudi B. Sediawan Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada, Jalan Grafika No. 2, Yogyakarta, Indonesia
Siti Syamsiah Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada, Jalan Grafika No. 2, Yogyakarta, Indonesia

DOI:

https://doi.org/10.15282/jmes.12.1.2018.18.0312

Keywords:

immobilization; mixed culture; alginate; activated carbon; beads; hydrogen.

Abstract

This paper presents an experimental investigation on using mixed culture for immobilization and co immobilization for hydrogen production. The shape and diameter of the beads were investigated. Hydrogen was produced from 10 g.L^-1glucose in anaerobic batch using immobilized mixed culture with extrusion dripping method. The alginate concentrations as immobilization material were 1%, 2%, and 3%. The mixed culture had three different biodigester sources consisting of cow dung, tofu waste, and fruit waste. The pretreatment of each mixed culture was acidification and enrichment. Then the mixed culture were mixed with immobilization material and inserted into a syringe, then dropped into 0.1M C_aCl₂. Activated carbon was added to alginate (coimmobilization) with ratio 1:1. The results showed that bead using 1% and 2% alginate concentrations were a pear-shaped. The highest concentration of hydrogen (molH₂/mol glucose) was 0.029 for immobilized beads with 2% alginate concentration and the lowest hydrogen (molH₂/mol glucose) was 0.009 for immobilized beads with 3% alginate concentration. Acetic acid was the most dominant. The highest VFA (mg.L^-1) was 695.85 for immobilized beads with 3% alginate concentration (acetic acid 271.49; propionic acid 163.33; isobutyrate acid 123.45; butyric acid 137.57). Most hydrogen was produced from 2% alginate concentration and spherical-shape.

References

Gomez-Flores M, Nakhla G, Hafez H. Hydrogen production and microbial kinetics of Clostridium termitidis in mono-culture and co-culture with Clostridium beijerinckii on cellulose. AMB Express. 2017;7:84.

Hallenbeck PC, Ghosh D, Skonieczny MT, Yargeau V. Microbiological and engineering aspects of biohydrogen production. Indian J Microbiol. 2009;49:48– 59.

Dufour J, Serrano DP, GaLvez JL, Moreno J, Garcı C. Life cycle assessment of processes for hydrogen production . Environmental feasibility and reduction of greenhouse gases emissions. Int J Hydrogen Energy. 2009;34:1370–6.

Kotay SM., Das D. Biohydrogen as a renewable energy resource — Prospects and potentials. Int J Hydrogen Energy. 2008;33:258–63.

Zhang L, Li J, Ban Q, He J, Jha AK. Metabolic pathways of hydrogen production in fermentative acidogenic microflora. J Microbiol Biotechnol. 2012;22:668–73.

Patel SKS, Kalia VC. Integrative Biological Hydrogen Production: An Overview. Indian J Microbiol. 2013;53:3–10.

Chandra R, Nikhil GN, Mohan VS. Single-stage operation of hybrid dark-photo fermentation to enhance biohydrogen production through regulation of system redox condition: Evaluation with real-field wastewater. Int J Mol Sci. 2015; 16:9540–56.

Hallenbeck PC, Hashesh MA, Ghosh D. Strategies for improving biological hydrogen production. Bioresour Technol. 2012;110:1–9.

Chen W, Chen S, Kumarkhanal S, Sung S. Kinetic study of biological hydrogen production by anaerobic fermentation. Int J Hydrogen Energy. 2006;31:2170–8.

Luque R, Campelo J, Clark J. Handbook of biofuels production: Processes and technologies. Woodhead Publishing Ltd.:Cambridge.pp.268; 2011.

Dong L, Zhenhong Y, Yongming S, Xiaoying K, Yu Z. Hydrogen production characteristics of the organic fraction of municipal solid wastes by anaerobic mixed culture fermentation. Int J Hydrogen Energy. 2009;34:812–20.

Amekan Y, Cahyanto MN, Sarto. Effect of Different Source of Inoculum to the Hydrogen Production from Melon Fruit Waste on Batch Fermentor. Conf. Proc. APCSLE CEAASC, Taipei, Taiwan. 2014, p. APCSLE – 342.

Chen CC, Lin CY, Lin MC. Acid – base enrichment enhances anaerobic hydrogen production process. Appl Microbiol Biotechnol. 2002;58:224–8. Cheong DY, Hansen CL. Feasibility Of Hydrogen Production From Anaerobic Mixed Fermentation. Trans ASABE. 2006;49:467–76.

Cheong DY, Hansen CL. Feasibility Of Hydrogen Production From Anaerobic Mixed Fermentation. Trans ASABE. 2006;49:467–76.

Hu B, Liu Y, Chi Z, Chen S. Biological Hydrogen Production Via Bacteria Immobilized In Calcium Alginate Gel Beads. Trans ASABE. 2008;1:25–37.

Marone A, Massini G, Patriarca C, Signorini A, Varrone C, Izzo G. Hydrogen production from vegetable waste by bioaugmentation of indigenous fermentative communities. Int J Hydrogen Energy. 2012;37:5612–22.

Sivagurunathan P, Sen B, Lin CY. Batch fermentative hydrogen production by enriched mixed culture: Combination strategy and their microbial composition. J Biosci Bioeng. 2014;117:222–8.

C-Y.Kumar G, Mudhoo A, Sivagurunathan P, Nagarajan D, Ghimire A, Lay C-H, et al. Recent insights into the cell immobilization technology applied for dark fermentative hydrogen production. Bioresour Technol. 2016;219:725–37.

Sekoai P., Awosusi AA, Yoro KO, Singo M, Oloye O, Ayeni AO, et al. Microbial cell immobilization in biohydrogen production: a short overview. Crit Rev Biotechnol. 2017;0:1–15.

Kumar A, Jain SR, Sharma CB, Joshi AP, Kalia VC. Increased H2 production microorganisms by immobilized. World J Mcrobiology Biotechnol. 1995;11:156– 9.

Wu KJ, Chang JS. Biohydrogen Production Using Suspended and Immobilized Mixed Microflora. J Chin Inst Chem Engrs. 2006;37:545–50.

Wu SY, Lin CN, Chang JS, Lee KS, Lin PJ. Microbial hydrogen production with immobilized sewage sludge. Biotechnol Prog. 2002;18:921–6.

Sekoai P, Yoro K, Daramola M. Batch Fermentative Biohydrogen Production Process Using Immobilized Anaerobic Sludge from Organic Solid Waste. Environments. 2016;3:38.

Dzionek A, Wojcieszyńska D, Guzik U. Natural carriers in bioremediation: A review. Electron J Biotechnol. 2016;23:28–36.

Merugu R, Rudra MPP, Nageshwari B, Rao AS, Ramesh D. Photoproduction of Hydrogen under Different Cultural Conditions by Alginate Immobilized Rhodopsedomonas palustris KU003. ISRN Renew Energy. 2012;2012:1–5.

Thakur V, Jadhav SK, Tiwari KL. Optimization of Different Parameters for Biohydrogen Production by Klebsiella oxytoca ATCC 13182. Trends Appl Sci Res. 2014;5:229–37.

Mesran MH, Mamat S, Pang YR, Hong TY, Muneera Z, Ghazali NFM, et al. Preliminary Studies on Immobilized Cells-Based Microbial Fuel Cell System on Its Power Generation Performance. J Asian Sci Res. 2014;4:428–35.

Azbar N, Kapdan IK. Use of Immobilized Cell Systems in Biohydrogen Production. In State of the Art and Progress in Production of Biohydrogen.Bentham Science Publishers.pp.231; 2012.

Al-Hajry HA, Al-Maskry SA, Al-Kharousi LM, El-Mardi O. Electrostatic Encapsulation and Growth of Plant Cell Cultures in Alginate. Biotechnol Prog. 1999;15:768–74.

Lee BB, Ravindra P, Chan ES. Size and Shape of Calcium Alginate Beads Produced by Extrusion Dripping. Chem Eng Technol. 2013:1627–42.

Li D. Encyclopedia of Microfluidics and Nanofluidics. Springer: USA. pp.402; 2008.

Jang D, Kim D, Moon J. Influence of fluid physical properties on ink-jet printability. Langmuir. 2009;25:2629–35.

Damayanti A, Sarto, Syamsiah S, Sediawan WB. Biohydrogen production from rotten orange with immobilized mixed culture: Effect of immobilization media for various composition of substrates. vol. 1699, In International Conference of Chemical and Material Engineering (ICCME), Semarang, Indonesia.pp. 030014-1– 7: AIP Publishing; 2015.

Willaert G.W. and Baron GV. Gel entrapment and micro-encapsulation: methods, applications and engineering principles. Willaert GW Baron, GV 1996;12:5–205.

Chan ES, Lee BB, Ravindra P, Poncelet D. Prediction models for shape and size of ca-alginate macrobeads produced through extrusion-dripping method. J Colloid Interface Sci. 2009;338:63–72.

Seifert DB, Phillips JA. Production of Small, Monodispersed Alginate Beads for Cell Immobilization. Biotechnol Prog. 1997;13:562–8.

Chen XH, Wang XT, Lou WY, Li Y, Wu H, Zong MH. Immobilization of Acetobacter sp . CCTCC M209061 for efficient asymmetric reduction of ketones and biocatalyst recycling. Microb Cell Fact. 2012;11:1–13.

Kong HJ, Yong K, Mooney DJ. Decoupling the dependence of rheological / mechanical properties of hydrogels from solids concentration. Polymer (Guildf). 2002;43:6239–46.

Karimi K. Lignocellulose- Based Bioproducts. Springer International Publishing:Switzerland.pp270; 2015.