Effect of Reaction Parameters on the Production of Cyclodextrin using Cyclodextrin Glucanotransferase from Bacillus licheniformis

  • Rohaida Che Man Department of Chemical Engineering, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
  • Shalyda Md. Shaarani@Nawi Department of Chemical Engineering, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
  • Zatul Iffah Mohd Arshad Faculty of Chemical and Process Engineering Technology, College of Engineering Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
  • Siti Kholijah Abdul Mudalip Department of Chemical Engineering, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia and Centre of Excellence for Advanced Research in Fluid Flow (CARIFF), Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
  • Siti Zubaidah Sulaiman Department of Chemical Engineering, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
  • Aizi Nor Mazila Ramli College of Computing and Applied Sciences, Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, Gambang, 26300 Gambang, Pahang, Malaysia.
Keywords: Bacillus licheniformis, cyclodextrin glucanotransferase, cyclodextrin, types of substrate, concentration of substrate, reaction time

Abstract

Cyclodextrin glucanotransferase (CGTase) is an industrial enzyme that converts starch into cyclodextrin (CD). CD has many potential applications in wide range of industries including food, cosmetic, pharmaceutical and plastic industries. Production of CD from CGTase is based on a few process parameters such as concentration of substrate, types of substrate, reaction time, pH and temperature. In this present study, the effect of types of substrate (corn, potato, wheat, rice and tapioca starch), concentration of substrate (1, 2, 3, 4, 5, 6, 7, 8 and 9%) and reaction time (1, 2, 3, 4 and 5 hr) on the CD production using commercial CGTase from Bacillus licheniformis were studied. Among the five types of substrate tested, the potato starch was the most suitable substrate for the production of CD with 10.24 mg/ml of CD. In addition, by using 6% (w/v) of potato starch, the production of CD was 15.16 mg/ml. The optimal reaction time was found to be 4 hr with 12.41 mg/ml of CD. Therefore, the best reaction parameters proved to be valuable for the highest CD production. Hence, CGTase from Bacillus licheniformis was demonstrated to produce high CD at optimum reaction conditions and it has potential to be used in large scale production for the industrial purposes.

Published
2020-10-07
Section
Articles