Effect of Processing Variables on Fish Oil Extraction

Authors

  • Nur Fathin Shamirah Daud Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300 Gambang, Pahang, Malaysia
  • Farhan Mohd Said Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300 Gambang, Pahang, Malaysia
  • Nur Hidayah Mat Yasin Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300 Gambang, Pahang, Malaysia
  • Naila Aishath The Maldives National University, Rehdebai Hingun, Machangolhi, Male’, 20371, Maldives

DOI:

https://doi.org/10.15282/jceib.v11i1.10934

Keywords:

Fish Waste, Soxhlet, Oleic acid, Biodiesel

Abstract

The accumulation of fish waste from seafood processing poses significant environmental and economic concerns. Therefore, there is a need to explore an efficient and sustainable extraction process for producing biodiesel, particularly through the extraction of fish oil rich in fatty acid methyl esters (FAME). This study investigated the effects of different processing variables, such as solvent type, temperature, and extraction time on the oil yield and FAME composition. The oil and FAME were extracted using a soxhlet extraction method. The optimal extraction conditions were ethanol, 60 °C, and 4 h, which yielded 49.0% oil yield with a high concentration of oleic acid (C18:1), ranging from 42% to 66%. These findings demonstrated that the oil extracted from fish waste has significant potential for cost-effective biodiesel production.

References

[1] Department of Fisheries Malaysia, “Annual Fisheries Statistics 2022,” 2023. Accessed: Jun. 17, 2025. [Online]. Available: https://ekkowunwkmj.exactdn.com/wp-content/uploads/2023/07/perangkaan-perikanan-2022-jilid-1-9.zip

[2] O. Villamil, H. Váquiro, and J. F. Solanilla, “Fish viscera protein hydrolysates: Production, potential applications and functional and bioactive properties,” Food Chemistry, vol. 224, pp. 160–171, 2017.

[3] S. Singh, T. Negi, N. A. Sagar, Y. Kumar, A. Tarafdar, R. Sirohi, et al., “Sustainable processes for treatment and management of seafood solid waste,” Science of the Total Environment, vol. 817, p. 152951, 2022.

[4] E. S. Okeke, R. E. Ita, E. J. Egong, L. E. Udofia, C. L. Mgbechidinma, and O. D. Akan, “Metaproteomics insights into fermented fish and vegetable products and associated microbes,” Food Chemistry: Molecular Sciences, vol. 3, p. 100045, 2021.

[5] D. Qin, S. Bi, X. You, M. Wang, X. Cong, C. Yuan, et al., “Development and application of fish scale wastes as versatile natural biomaterials,” Chemical Engineering Journal, vol. 428, p. 131102, 2022.

[6] P. Araujo, C. Truzzi, I. Belghit, and M. Antonucci, “The impact of seawater warming on fatty acid composition and nutritional quality indices of Trematomus bernacchii from the Antarctic region,” Food Chemistry, vol. 365, p. 130500, 2021.

[7] S. Singh, T. Negi, N. A. Sagar, Y. Kumar, A. Tarafdar, R. Sirohi, et al., “Sustainable processes for treatment and management of seafood solid waste,” Science of the Total Environment, vol. 817, p. 152951, 2022.

[8] C. L. Mgbechidinma, G. Zheng, E. B. Baguya, H. Zhou, S. U. Okon, and C. Zhang, “Fatty acid composition and nutritional analysis of waste crude fish oil obtained by optimized milder extraction methods,” Environmental Engineering Research, vol. 28, no. 2, p. 220034, 2022.

[9] L. Inguglia, M. Chiaramonte, V. Di Stefano, D. Schillaci, G. Cammilleri, L. Pantano, et al., “Salmo salar fish waste oil: Fatty acids composition and antibacterial activity,” PeerJ, vol. 8, p. e9299, 2020.

[10] R. Yahyaee, B. Ghobadian, and G. Najafi, “Waste fish oil biodiesel as a source of renewable fuel in Iran,” Renewable and Sustainable Energy Reviews, vol. 17. pp. 312–319, 2013.

[11] S. Al Azad, L. Y. Wuen, and M. T. Bin Mohamad Lal, “Potential of fish wastes as feedstock for biodiesel,” Advances in Bioscience and Biotechnology, vol. 10, no. 05, pp. 109–118, 2019, doi: 10.4236/abb.2019.105008.

[12] A. F. Samat, N. A. Safiah Muhamad, N. A. Abd Rasib, S. A. Mohd Hassan, K. S. Ahmad Sohaimi, and N. I. Iberahim, “The potential of biodiesel production derived from fish waste,” in IOP Conference Series: Materials Science and Engineering, vol. 318, no. 1, p. 012017, 2018.

[13] K. Kara, F. Ouanji, E. M. Lotfi, M. El Mahi, M. Kacimi, and M. Ziyad, “Biodiesel production from waste fish oil with high free fatty acid content from Moroccan fish-processing industries,” Egyptian Journal of Petroleum, vol. 27, no. 2, pp. 249–255, 2018.

[14] T. Zhang, B. Du, Y. Lin, M. Zhang, and Y. Liu, “Production of biodiesel and high-protein feed from fish processing wastes using in situ transesterification,” Molecules, vol. 25, no. 7, p. 1650, 2020.

[15] A. B. Fadhil, E. T. B. Al-Tikrity, and M. A. Albadree, “Transesterification of a novel feedstock, Cyprinus carpio fish oil: Influence of co-solvent and characterization of biodiesel,” Fuel, vol. 162, pp. 215–223, 2015.

[16] G. F. Smaisim, N. M. Prabu, S. A P, and A. M. Abed, “Synthesis of biodiesel from fish processing waste by nano magnetic catalyst and its thermodynamic analysis,” Case Studies in Thermal Engineering, vol. 35, 2022.

[17] E. Sathiyamoorthi, J. Lee, M. F. Albeshr, M. D. Ramesh, and K. Brindhadevi, “Effect of solar powered MgO/graphene nano catalysed biodiesel production from Scomber scombrus,” Environmental Research, vol. 258, p. 119407, 2024.

[18] N. Rahman, S. Hashem, S. Akther, and J. S. Jothi, “Impact of various extraction methods on fatty acid profile, physicochemical properties, and nutritional quality index of Pangus fish oil,” Food Science & Nutrition, vol. 11, no. 8, pp. 4688–4699, 2023.

[19] M. Á. Rincón-Cervera, M. B. Villarreal-Rubio, R. Valenzuela, and A. Valenzuela, “Comparison of fatty acid profiles of dried and raw by-products from cultured and wild fishes,” European Journal of Lipid Science and Technology, vol. 119, no. 9, p. 1600516, 2017.

[20] M. Haq, R. Ahmed, Y. J. Cho, and B. S. Chun, “Quality properties and bio-potentiality of edible oils from Atlantic salmon by-products extracted by supercritial carbon dioxide and conventional methods,” Waste Biomass Valorization, vol. 8, no. 6, pp. 1953–1967, 2017.

[21] S. Suhararamli, M. Yazid, A. Manap, M. Zaidul, I. Sarker, and S. N. Mohamed, “Comparison studies of fatty acids profiles extracted from selected Malaysian seawater and freshwater fish wastes,” International Journal of Engineering and Advanced Technology, pp. 149-154, 2016.

[22] P. Ideia, J. Pinto, R. Ferreira, L. Figueiredo, V. Spínola, and P. C. Castilho, “Fish processing industry residues: A review of valuable products extraction and characterization methods,” Waste and Biomass Valorization, vol. 11, no. 7, pp. 3223–3246, 2020.

[23] D. Madhu, R. Arora, S. Sahani, V. Singh, and Y. C. Sharma, “Synthesis of high-quality biodiesel using feedstock and catalyst derived from fish wastes,” Journal of Agricultural and Food Chemistry, vol. 65, no. 10, pp. 2100–2109, 2017.

[24] F. M. Said, N. S. M. Rapidi, N. S. M. Ibrahim, N. H. M. Yasin, and N. F. S. Daud, “Effect of processing parameters to the fish oil from fish waste via modified soxhlet extraction,” ASM Science Journal, vol. 17, pp. 1-7, 2022.

[25] B. A. Adejumo, A. T. Alakowe, and D. E. Obi, “Effect of heat treatment on the characteristics and oil yield of Moringa oleifera seeds,” International Journal of Engineering Science, vol. 2, no. 1, pp. 232–239, 2013.

[26] M. Gaikwad, K. Shantanu, B. Snehal, U. Vaibhav, and R. Amol, “Extraction, characterization and comparison of fixed oil of Moringa oleifera L & Moringa concanensis Nimmo Fam. Moringaceae,” International Journal of PharmTech Research, vol. 3, no. 3, pp. 1567–1575, 2011.

[27] S. Ferdosh, M. Z. I. Sarker, N. N. N. A. Rahman, M. J. H. Akanda, K. Ghafoor, and M. O. A. Kadir, “Simultaneous extraction and fractionation of fish oil from tuna by-product using supercritical carbon dioxide (SC-CO2),” Journal of Aquatic Food Product Technology, vol. 25, no. 2, pp. 230–239, 2016.

[28] M. Mohsen-Nia, H. Amiri, and B. Jazi, “Dielectric constants of water, methanol, ethanol, butanol and acetone: Measurement and computational study,” Journal of Solution Chemistry, vol. 39, no. 5, pp. 701–708, 2010.

[29] N. V Sastry and M. K. Valand, “Dielectric constants, refractive indexes and polarizations for 1-alcohol + heptane mixtures at 298.15 and 308.15 K,” Berichte der Bunsengesellschaft für physikalische Chemie, vol. 101, no. 2, pp. 243-250, 1997.

[30] A. C. Kumoro, D. H. Wardhani, T. D. Kusworo, M. Djaeni, T. C. Ping, and Y. Ma’rifat Fajar Azis, “Fish protein concentrate for human consumption: A review of its preparation by solvent extraction methods and potential for food applications,” Annals of Agricultural Sciences, vol. 67, no. 1, pp. 42–59, 2022.

[31] A. E. Ghaly, V. V. Ramakrishnan, M. S. Brooks, S. M. Budge, and D. Dave, “Fish processing wastes as a potential source of proteins, amino acids and oils: A critical review,” Amino acids and oils: A critical review. Journal of Microbial and Biochemical Technology, vol. 5, no. 4, pp. 107–129, 2013.

[32] T. Kraiem, A. Ben Hassen-Trabelsi, S. Naoui, H. Belayouni, and M. Jeguirim, “Characterization of the liquid products obtained from Tunisian waste fish fats using the pyrolysis process,” Fuel Processing Technology, vol. 138, pp. 404–412, 2015.

[33] J. F. Costa, M. F. Almeida, M. C. M. Alvim-Ferraz, and J. M. Dias, “Biodiesel production using oil from fish canning industry wastes,” Energy Conversion and Management, vol. 74, pp. 17–23, 2013.

[34] S. Shamsudin and J. Salimon, “Physicochemical characteristics of aji-aji fish seriola nigrofasciata lipids,” Malaysia Journal of Analytical Sciences, vol. 10, no. 1, pp. 55-58, 2006.

[35] U. Rashid, F. Anwar, B. R. Moser, and G. Knothe, “Moringa oleifera oil: A possible source of biodiesel,” Bioresource Technology, vol. 99, no. 17, pp. 8175–8179, 2008.

[36] A. Nosheen, R. Naz, A. T. Tahir, H. Yasmin, R. Keyani, B. Mitrevski, et al., “Improvement of safflower oil quality for biodiesel production by integrated application of PGPR under reduced amount of NP fertilizers,” PLoS One, vol. 13, no. 8, 2018.

[37] N. Shahi, S. Goutam, R. Thakur, A. Singh, and U. Lohani, “Effect of process parameters on oil yield in process optimization for extraction of essential fatty acid from fish using solvent extraction,” Current Journal of Applied Science and Technology, vol. 25, no. 3, pp. 1–12, 2018.

[38] The American Oil Chemists' Society, “Official methods and recommended practices of the American Oil Chemists’ Society,” D. Firestone, Ed., 5th edition, USA, 1998.

[39] C. S. Eskilsson and E. Bjorklund, “Analytical-scale microwave-assisted extraction,” Journal of Chromatography A, vol. 902, no. 1, pp. 227-250, 2000.

[40] T. H. Wu and P. J. Bechtel, “Salmon by-product storage and oil extraction,” Food Chemistry, vol. 111, no. 4, pp. 868–871, 2008.

[41] Q. Ghazali and N. H. M. Yasin, “The effect of organic solvent, temperature and mixing time on the production of oil from Moringa oleifera seeds,” in IOP Conference Series: Earth and Environmental Science, vol. 36, no. 1, pp. 868-871, 2016.

[42] S. Mani, S. Jaya, and R. Vadivambal, “Optimization of solvent extraction of moringa (Moringa Oleifera) seed kernel oil using response surface methodology,” Food and Bioproducts Processing, vol. 85, no. 4C, pp. 328–335, 2007.

[43] A. Głowacz-Różyńska, M. Tynek, E. Malinowska-Pańczyk, D. Martysiak-Żurowska, R. Pawłowicz, and I. Kołodziejska, “Comparison of oil yield and quality obtained by different extraction procedures from salmon (Salmo salar) processing byproducts,” European Journal of Lipid Science and Technology, vol. 118, no. 11, pp. 1759–1767, 2016.

[44] E. Choe and D. B. Min, “Mechanisms and factors for edible oil oxidation,” Comprehensive Reviews in Food Science and Food Safety, vol. 5, no. 4, pp. 169–186, 2006.

[45] N. H. Rushan, N. H. Mat Yasin, and F. M. Said, “The effect of culture medium on the oil yield and fatty acid methyl ester of freshwater microalgae Chlorella vulgaris,” Chemical Engineering Communications, vol. 208, no. 4, pp. 592–600, 2021.

[46] M. J. Ramos, C. M. Fernández, A. Casas, L. Rodríguez, and Á. Pérez, “Influence of fatty acid composition of raw materials on biodiesel properties,” Bioresource Technology, vol. 100, no. 1, pp. 261–268, 2009.

[47] A. S. Ramadhas, S. Jayaraj, and C. Muraleedharan, “Biodiesel production from high FFA rubber seed oil,” Fuel, vol. 84, no. 4, pp. 335–340, 2005.

[48] R. S. Menezes, A. T. Soares, J. G. Marques Júnior, R. G. Lopes, R. F. da Arantes, R. B. Derner, et al., “Culture medium influence on growth, fatty acid, and pigment composition of Choricystis minor var. minor: A suitable microalga for biodiesel production,” Journal of Applied Phycology, vol. 28, no. 5, pp. 2679–2686, 2016.

Downloads

Published

2025-07-14

Issue

Section

Articles

How to Cite

1.
Daud NFS, Mohd Said F, Mat Yasin NH, Aishath N. Effect of Processing Variables on Fish Oil Extraction. J. Chem. Eng. Ind. Biotechnol. [Internet]. 2025 Jul. 14 [cited 2025 Jul. 30];11(1):33-40. Available from: https://journal.ump.edu.my/jceib/article/view/10934

Similar Articles

11-20 of 37

You may also start an advanced similarity search for this article.