EXTRACTION AND MICROENCAPSULATION OF POLYPHENOLS FROM ORTHOSIPHON STAMINEUS LEAVES

S.F.  Pang; M.M. Yusoff; J. Gimbun

doi:10.15282/jmes.7.2014.2.0100

Authors

S.F. Pang Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, 26300 Gambang, Pahang, Malaysia
M.M. Yusoff Faculty of Industrial Science and Technology, Universiti Malaysia Pahang, 26300 Gambang, Pahang, Malaysia
J. Gimbun Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, 26300 Gambang, Pahang, Malaysia

DOI:

https://doi.org/10.15282/jmes.7.2014.2.0100

Keywords:

Extraction; microencapsulation; misai kucing; eupatorin; sinensetin; rosmarinic acid.

Abstract

This paper presents an extraction and microencapsulation study of polyphenols obtained from Orthosiphon stamineus leaves. Extraction using aqueous solvent enabled a simultaneous extraction of both hydrophilic and lipophilic phenolic compounds. Higher solid concentration leads to higher solution viscosity, bigger particle size, lower moisture content and a less dented surface, which may improve particle flowability. The results suggest that eupatorin and rosmarinic acid are more susceptible to thermal degradation than sinensetin during spray drying. The formulation of 1:9 whey protein to maltodextrin ratio was also found to be more effective, with 86.7% total flavonoids retention compared to other formulations, viz, 1:1 and 9:1.

References

Ho C-H, Noryati I, Sulaiman S-F, Rosma A. In vitro antibacterial and antioxidant activities of Orthosiphon stamineus Benth. extracts against food- borne bacteria. Food Chemistry. 2010;122:1168-72.

Arafat O, Tham S, Sadikun A, Zhari I, Haughton P, Asmawi M. Studies on diuretic and hypouricemic effects of Orthosiphon stamineus methanol extracts in rats. Journal of Ethnopharmacology. 2008;118:354-60.

Mohamed EAH, Yam MF, Ang LF, Mohamed AJ, Asmawi MZ. Antidiabetic Properties and Mechanism of Action of Orthosiphon stamineus Benth Bioactive Sub-fraction in Streptozotocin-induced Diabetic Rats. Journal of Acupuncture and Meridian Studies. 2013;6:31-40.

Dolečková I, Rárová L, Grúz J, Vondrusová M, Strnad M, Kryštof V. Antiproliferative and antiangiogenic effects of flavone eupatorin, an active constituent of chloroform extract of Orthosiphon stamineus leaves. Fitoterapia. 2012;83:1000-7.

Akowuah G, Ismail Z, Norhayati I, Sadikun A. The effects of different extraction solvents of varying polarities on polyphenols of Orthosiphon stamineus and evaluation of the free radical-scavenging activity. Food Chemistry. 2005;93:311-7.

Pouralinazar F, Yunus MAC, Zahedi G. Pressurized liquid extraction of Orthosiphon stamineus oil: Experimental and modeling studies. The Journal of Supercritical Fluids. 2012;62:88-95.

Tabaraki R, Nateghi A. Optimization of ultrasonic-assisted extraction of natural antioxidants from rice bran using response surface methodology. Ultrasonics Sonochemistry. 2011;18:1279-86.

Zhang H-F, Yang X-H, Zhao L-D, Wang Y. Ultrasonic-assisted extraction of epimedin C from fresh leaves of Epimedium and extraction mechanism. Innovative Food Science & Emerging Technologies. 2009;10:54-60.

Ncube N, Afolayan A, Okoh A. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African Journal of biotechnology. 2008;7:1797-806.

Pang SF, Yusoff MM, Gimbun J. Assessment of phenolic compounds stability and retention during spray drying of Orthosiphon stamineus extracts. Food Hydrocolloids. 2014;37:159-65.

Akowuah GA, Zhari I. Effect of extraction temperature on stability of major polyphenols and antioxidant activity of Orthosiphon stamineus leaf. Journal of herbs, spices & medicinal plants. 2010;16:160-6.

Al-Kayiem HH, Md Yunus Y. Drying of empty fruit bunches as wasted biomass by hybrid solar–thermal drying technique. Journal of Mechanical Engineering and Sciences. 2013;5:652-61.

Xie YL, Zhou HM, Liang XH, He BS, Han XX. Study on the morphology, particle size and thermal properties of vitamin A microencapsulated by starchoctenylsucciniate. Agricultural Sciences in China. 2010;9:1058-64.

Betz M, Steiner B, Schantz M, Oidtmann J, Mäder K, Richling E, et al. Antioxidant capacity of bilberry extract microencapsulated in whey protein hydrogels. Food Research International. 2012;47:51-7.

Şahin-Nadeem H, Dinçer C, Torun M, Topuz A, Özdemir F. Influence of inlet air temperature and carrier material on the production of instant soluble sage (Salvia fruticosa Miller) by spray drying. LWT-Food Science and Technology. 2013;52:31-8.

Farahany S, Bakhsheshi-Rad HR, Idris MH, Abdul Kadir MR, Lotfabadi AF, Ourdjini A. In-situ thermal analysis and macroscopical characterization of Mg–xCa and Mg–0.5Ca–xZn alloy systems. Thermochimica Acta. 2012;527:180-9.

Pang S, Yusoff M, Gimbun J. Assessment on bioavailability of flavonoids from orthosiphon stamineus during spray drying. Developments in Sustainable Chemical and Bioprocess Technology. 2013:137-44.

Wach A, Pyrzyńska K, Biesaga M. Quercetin content in some food and herbal samples. Food Chemistry. 2007;100:699-704.

Buchner N, Krumbein A, Rohn S, Kroh LW. Effect of thermal processing on the flavonols rutin and quercetin. Rapid Communications in Mass Spectrometry. 2006;20:3229-35.

Young S, Sarda X, Rosenberg M. Microencapsulating properties of whey proteins. 2. Combination of whey proteins with carbohydrates. Journal of Dairy Science. 1993;76:2878-85.

Choi K-O, Ryu J, Kwak H-S, Ko S. Spray-dried conjugated linoleic acid encapsulated with Maillard reaction products of whey proteins and maltodextrin. Food Science and Biotechnology. 2010;19:957-65.

Anandharamakrishnan C, Rielly CD, Stapley A. Loss of solubility of α-lactalbumin and β-lactoglobulin during the spray drying of whey proteins. LWTFood Science and Technology. 2008;41:270-7.

Rosenberg M, Sheu T. Microencapsulation of volatiles by spray-drying in whey protein-based wall systems. International Dairy Journal. 1996;6:273-84.