Isolation and screening of pectinase producers from fruit wastes
DOI:
https://doi.org/10.15282/cst.v5i1.13087Keywords:
Agricultural Biomass, Isolation & Screening, Enzyme production, PectinaseAbstract
This study aimed to identify, screen and characterize pectinase-producing bacteria from fruit waste for potential use in the biotechnology sector. Three isolated bacterial strains were tested for their pectinase production using both commercial pectin and fruit waste substrates, specifically pineapple and banana peels, alongside an assessment of their growth characteristics. Among the three isolates, strain M1 exhibited the best performance in terms of growth and enzyme activity. When commercial citrus pectin was used as the substrate, M1 achieved the highest pectinase activity (8.15 U/mL), followed by B1 (7.423 U/mL) and P2 (7.035 U/mL). When fruit waste was used as the substrate, M1 again produced the highest amount of pectinase. Notably, pineapple peel yielded slightly higher pectinase activity for M1 (7.38 U/mL) compared to banana peel (7.032 U/mL), indicating that pineapple peel is a better inducer of pectinase enzyme production. In terms of growth dynamics, M1 also recorded the highest specific growth rate and the shortest doubling time across all substrates, reinforcing its potential as the most efficient pectinase producer. These findings suggest that strain M1, especially when cultivated on pineapple peel waste, holds significant promise for cost-effective and sustainable industrial pectinase production, contributing to the development of environmentally friendly bioprocesses.
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