TWO LEVEL FACTORIAL SCREENING OF MICROWAVE-ASSISTED EXTRACTION PARAMETERS FOR THE RECOVERY OF PHENOLIC COMPOUNDS FROM VERNONIA CINEREA LEAF

  • Oluwaseun Ruth Alara
  • Nour Hamid Abdurahman
  • John Adewole Alara
Keywords: Two-level factorial design, Total flavonoid content, Total phenolic content, Vernonia cinerea, Screening

Abstract

Vernonia cinerea is one of the medicinal plants with several potentials for treating different ailments. In the present study, Microwave-assisted extraction (MAE) was employed in extracting phenolics compounds from this plant. However, different factors that affect this extraction method in the recovery of phenolics compounds abound, these factors need to be screened to determine actual contributing factor in order to minimize cost. Irradiation time (1-5 min), ethanol concentration (20-60% v/v), microwave power (40-80 W), extraction temperature (40-80 oC), and feed/solvent (1:10 - 1:18 g/mL) have been screened using two-factorial design for the recoveries of phenolic compounds from V. cinerea leaves. The results obtained in this study indicated that only microwave power, ethanol concentration, irradiation time and feed/solvent contributed to recoveries of total phenolic content (TPC) and total flavonoid content (TFC) from V. cinerea leaves. Thus, these factors at these ranges can be further optimized to obtain optimal yields of phenolic compounds from V. cinerea leaves.

Published
2019-03-01
Section
Articles