Binding studies of ruthenium (II) complexes with DNA isolated from pea (Pisumsativum) extract
DOI:
https://doi.org/10.15282/cst.v5i1.12229Keywords:
Pea (Pisum sativum), Binding studies, Ruthenium complex, Polypyridyl ligand, Benesi-Hildebrand constantAbstract
The increasing impact of group 8–10 metal organometallic complexes academically and industrially over the years demonstrates remarkable progress in harvesting useful reagents, catalysts, and chemotherapeutic agents. Recently, these complexes have been widely analyzed for their photophysical properties and medical applications as they efficiently treat different types of cancer through photodynamic therapy (PDT). Understanding the interaction of bipyridyl complexes with specific pea(Pisumsativum)genes could show how these metal complexes influence plant traits. Peas, mainly through their genetic modifications, show promise in cancer treatment by serving as bioreactors for producing therapeutic antibodies. The pea genome contains a large number of genes involved in various biological processes, including those responsible for plant growth, development, and stress responses. Ru (II) polypyridyl complexes have shown excellent DNA binding results. These complexes bind to DNA through the intercalative mode. The binding of Ru(II) polypyridyl complex [RuL3]2+ (where L= bpy, dmbpy) with DNA of pea extracts has been studied in an aqueous medium through absorption and emission spectral techniques. The binding constant (Kb) for these reactions is determined from the Benesi-Hildebrand equation using both the absorption intensity data and emission studies.
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