From Preparation to Product: Factors Influencing Probiotic Viability in Spray Drying
DOI:
https://doi.org/10.15282/cst.v4i1.11125Keywords:
dried state, probiotics, spray-drying, viabilityAbstract
With growing health awareness, particularly amid the SARS-CoV-2 pandemic, consumers increasingly value nutrition, diet, and food safety. Probiotic-based foods and beverages are widely recognized for their health benefits, including improved gut health and immune function. Spray drying is a scalable and efficient method for encapsulating, enhancing the stability and shelf life of probiotics. This review explores strategies to optimize the spray drying process, with a particular focus on factors influencing probiotic viability during and after drying. Key considerations included strain-specific thermal tolerance, feed composition, and critical process parameters such as drying temperature and feed rate. Notably, encapsulating agents play a vital role in maintaining the physicochemical quality of the final product while protecting probiotics from environmental stress. Recent advancements in encapsulation technologies, including biopolymers, hybrid materials, and emerging nanotechnology-based solutions have shown significant potential for enhancing probiotic survival under harsh processing conditions. Future research should integrate molecular-level insights, such as omics-based approaches, to better understand stress responses and optimize encapsulation strategies. Genome-editing tools and high-throughput screening techniques could accelerate the creation of thermotolerant probiotic strains, enabling more robust formulations. In parallel, the development of environmentally sustainable encapsulating agents with superior protective properties is essential to advance both efficiency and scalability. By addressing these challenges, spray drying can be further refined to produce durable, high-quality probiotic formulations that meet the growing demand for functional foods and beverages, while aligning with evolving consumer health priorities.
References
[1] R. Rajam and P. Subramanian, “Encapsulation of probiotics: past, present and future,” Beni-Suef University Journal of Basic and Applied Sciences, vol. 11, no. 1, p. 46, 2022.
[2] M. H. Abd El-Salam and S. El-Shibiny, “Preparation and properties of milk proteins-based encapsulated probiotics: A review,” Dairy Science & Technology, vol. 95, no. 4, pp. 393–412, 2015.
[3] S. S. Mishra, U. Das, R. Biswal, and S. S. Behera, “Probiotic beverages in India: History and current developments,” Probiotic Beverages, pp. 9–33, 2021.
[4] D. Mazzantini, M. Calvigioni, F. Celandroni, A. Lupetti, and E. Ghelardi, “Spotlight on the compositional quality of probiotic formulations marketed worldwide,” Frontiers in Microbiology, vol. 12, pp. 1–16, 2021.
[5] P. G. D. A. Brasiel, T. Costa de Almeida, K. Mateus, A. Fernandes de Carvalho, S. C. Potente Dutra Luquetti, and M. D. C. Gouveia Peluzio, “Maintenance of probiotic characteristics of dry kefir: Is it possible?” Journal of Culinary Science & Technology, vol. 20, no. 5, pp. 463-472, 2020.
[6] M. Sharifi, A. Moridnia, D. Mortazavi, M. Salehi, M. Bagheri, and A. Sheikhi, “Kefir: A powerful probiotics with anticancer properties,” Medical Oncology, vol. 34, no. 11, p. 183, 2017.
[7] J. Van Wyk, “Kefir: The champagne of fermented beverages,” in Fermented Beverages, Elsevier, pp. 473–527, 2019.
[8] I. Atalar and M. Dervisoglu, “Optimization of spray drying process parameters for kefir powder using response surface methodology,” LWT-Food Science and Technology, vol. 60, no. 2, pp. 751–757, 2015.
[9] M. Teijeiro, P. F. Pérez, G. L. De Antoni, and M. A. Golowczyc, “Suitability of kefir powder production using spray drying,” Food Research International, vol. 112, pp. 169–174, 2018.
[10] O. Gul and I. Atalar, “Different stress tolerance of spray and freeze dried Lactobacillus casei Shirota microcapsules with different encapsulating agents,” Food Science and Biotechnology, vol. 28, no. 3, pp. 807–816, 2019.
[11] M. A. Golowczyc, C. L. Gerez, J. Silva, A. G. Abraham, G. L. De Antoni, and P. Teixeira, “Survival of spray-dried Lactobacillus kefir is affected by different protectants and storage conditions,” Biotechnology Letters, vol. 33, no. 4, pp. 681–686, 2011.
[12] G. Broeckx, D. Vandenheuvel, I. J. J. Claes, S. Lebeer, and F. Kiekens, “Drying techniques of probiotic bacteria as an important step towards the development of novel pharmabiotics,” International Journal of Pharmaceutics, vol. 505, pp. 303–318, 2016.
[13] P. Schuck, R. Jeantet, B. Bhandari, X. D. Chen, Í. T. Perrone, A. F. de Carvalho et al., “Recent advances in spray drying relevant to the dairy industry: A comprehensive critical review,” Drying Technology, vol. 34, no. 15, pp. 1773–1790, 2016.
[14] S. H. Peighambardoust, A. Golshan Tafti, and J. Hesari, “Application of spray drying for preservation of lactic acid starter cultures: A review,” Trends in Food Science & Technology, vol. 22, no. 5, pp. 215–224, 2011.
[15] D. Santos, A. C. Maurício, V. Sencadas, J. D. Santos, M. H. Fernandes, and P. S. Gomes, “Spray drying: An overview,” Biomaterials-Physics and Chemistry-New Edition, vol. 2, pp. 9-35, 2018.
[16] A. P. van Boven, A. Dubbelboer, T. J. A. Janssen, J. Schröder, J. J. W. Sewalt, R. Kohlus et al., “Investigating the impact of air distribution on spray dryer operability using CFD simulations and pilot-scale experiments,” Powder Technology, vol. 440, p. 119779, 2024.
[17] S. C. Samantha, A. S. M. Bruna, R. M. Adriana, B. Fabio, A. R. Sandro, and R. C. A. Aline, “Drying by spray drying in the food industry: Micro-encapsulation, process parameters and main carriers used,” African Journal of Food Science, vol. 9, no. 9, pp. 462–470, 2015.
[18] D. Arepally, R. S. Reddy, and T. K. Goswami, “Encapsulation of: Lactobacillus acidophilus NCDC 016 cells by spray drying: Characterization, survival after in vitro digestion, and storage stability,” Food & Function, vol. 11, no. 10, pp. 8694–8706, 2020.
[19] C. Soukoulis, S. Behboudi-Jobbehdar, L. Yonekura, C. Parmenter, and I. Fisk, “Impact of milk protein type on the viability and storage stability of microencapsulated Lactobacillus acidophilus NCIMB 701748 using spray drying,” Food and Bioprocess Technology, vol. 7, no. 5, pp. 1255–1268, 2014.
[20] D. Arepally, R. S. Reddy, and T. K. Goswami, “Studies on survivability, storage stability of encapsulated spray dried probiotic powder,” Current Research Food Science, vol. 3, pp. 235–242, 2020.
[21] R. Paéz, L. Lavari, G. Vinderola, G. Audero, A. Cuatrin, N. Zaritzky, and J. Reinheimer, “Effect of heat treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion,” Food Research International, vol. 48, no. 2, pp. 748–754, 2012.
[22] J. Silva, R. Freixo, P. Gibbs, and P. Teixeira, “Spray-drying for the production of dried cultures,” International Journal of Dairy Technology, vol. 64, no. 3, pp. 321–335, 2011.
[23] S. Huang et al., “Spray drying of probiotics and other food-grade bacteria: A review,” Trends in Food Science & Technology, vol. 63, pp. 1–17, 2017.
[24] Y. Zhang, J. Lin, and Q. Zhong, “Effects of media, heat adaptation, and outlet temperature on the survival of Lactobacillus salivarius NRRL B-30514 after spray drying and subsequent storage,” LWT - Food Science Technology, vol. 74, pp. 441–447, 2016.
[25] Q. Zheng, G. Shu, J. Kou, X. Cui, and J. Meng, “The effect of eight thermal protectants on the survival rate and the viable counts of Lactobacillus casei after heat treatment in fermented goat milk,” Acta Universitatis Cinbinesis, Series E: Food Technology, vol. 23, no. 1, pp. 3–10, 2019.
[26] F. Kaymak Ertekin, Ö. Köprüalan Aydın, and Ö. Altay, “Enhancing viability of Lactobacillus plantarum BG24 through optimized spray drying: insights into process parameters, carrier agents, comparative analysis with freeze drying, and storage condition influences,” Food Science & Nutrition, pp. 1–17, 2024.
[27] F. Hao, N. Fu, H. Ndiaye, M. W. Woo, R. Jeantet, and X. D. Chen, “Thermotolerance, survival, and stability of lactic acid bacteria after spray drying as affected by the increase of growth temperature,” Food Bioprocess Technology, vol. 14, no. 1, pp. 120–132, 2021.
[28] M. Jantzen, A. Göpel, and C. Beermann, “Direct spray drying and microencapsulation of probiotic Lactobacillus reuteri from slurry fermentation with whey,” Journal of Applied Microbiology, vol. 115, no. 4, pp. 1029–1036, 2013.
[29] G. Broeckx, S. Kiekens, K. Jokicevic, E. Byl, T. Henkens, D. Vandenheuvel et al., “Effects of initial cell concentration, growth phase, and process parameters on the viability of Lactobacillus rhamnosus GG after spray drying,” Drying Technology, vol. 38, no. 11, pp. 1474–1492, 2020.
[30] Y. Wang, F. Hao, W. Lu, X. Suo, E. Bellenger, N. Fu et al., “Enhanced thermal stability of lactic acid bacteria during spray drying by intracellular accumulation of calcium,” Journal of Food Engineering, vol. 279, p. 109975, 2020.
[31] X. Gao, J. Kong, H. Zhu, B. Mao, S. Cui, and J. Zhao, “Lactobacillus, Bifidobacterium and Lactococcus response to environmental stress: Mechanisms and application of cross-protection to improve resistance against freeze-drying,” Journal of Applied Microbiology, vol. 132, no. 2, pp. 802–821, 2022.
[32] V. A. Veselovsky, M. S. Dyachkova, D. A. Bespiatykh, R. A. Yunes, E. A. Shitikov, P. S. Polyaeva, et al., “The gene expression profile differs in growth phases of the Bifidobacterium longum culture,” Microorganisms, vol. 10, no. 8, p. 1683, 2022.
[33] G. Liu, H. Chang, Y. Qiao, K. Huang, A. Zhang, Y. Zhao et al., “Profiles of small regulatory RNAs at different growth phases of Streptococcus thermophilus during pH-controlled batch fermentation,” Frontiers in Microbiology, vol. 12, p.765144, 2021.
[34] A. Y. Bustos, M. P. Taranto, C. L. Gerez, S. Agriopoulou, S. Smaoui, T. Varzakas et al., “Recent advances in the understanding of stress resistance mechanisms in probiotics: relevance for the design of functional food systems,” Probiotics and Antimicrobial Proteins, vol. 17, no. 1, 138-158, 2024.
[35] S. Settachaimongkon, H. J. F van Valenberg, V. Winata, X. Wang, M. J. R. Nout et al., “Effect of sublethal preculturing on the survival of probiotics and metabolite formation in set-yoghurt,” Food Microbiology, vol. 49, pp. 104–115, 2015.
[36] J. Bommasamudram, A. Muthu, and S. Devappa, “Effect of sub-lethal heat stress on viability of Lacticaseibacillus casei N in spray-dried powders,” LWT - Food Science Technology, vol. 155, p. 112904, 2022.
[37] C. Desmond, C. Stanton, G. F. Fitzgerald, K. Collins, and R. Paul Ross, “Environmental adaptation of probiotic lactobacilli towards improvement of performance during spray drying,” International Dairy Journal, vol. 12, no. 2–3, pp. 183–190, 2002.
[38] Z. Shokri, M. R. Fazeli, M. Ardjmand, S. M. Mousavi, and K. Gilani, “Factors affecting viability of Bifidobacterium bifidum during spray drying,” DARU Journal of Pharmaceutical Sciences, vol. 23, no. 1, pp. 1-9, 2015.
[39] M.-J. Chen, H.-Y. Tang, and M.-L. Chiang, “Effects of heat, cold, acid and bile salt adaptations on the stress tolerance and protein expression of kefir-isolated probiotic Lactobacillus kefiranofaciens M1,” Food Microbioogy, vol. 66, pp. 20–27, 2017.
[40] K. Anekella and V. Orsat, “Optimization of microencapsulation of probiotics in raspberry juice by spray drying,” LWT - Food Science Technology, vol. 50, no. 1, pp. 17–24, 2013.
[41] A. Ghandi, I. B. Powell, X. D. Chen, and B. Adhikari, “The effect of dryer inlet and outlet air temperatures and protectant solids on the survival of Lactococcus lactis during spray drying,” Drying Technology, vol. 30, no. 14, pp. 1649–1657, 2012.
[42] M. Chavarri, I. Maranon, and M. Carmen, “Encapsulation technology to protect probiotic bacteria,” In Probiotics. IntechOpen, 2012.
[43] H. S. El-Sayed, H. H. Salama, and A. E. Edris, “Survival of Lactobacillus helveticus CNRZ32 in spray dried functional yogurt powder during processing and storage,” Journal of the Saudi Society of Agricultural Sciences, vol. 19, no. 7, pp. 461–467, 2020.
[44] M. Aponte, G. D. Troianiello, M. Di Capua, R. Romano, and G. Blaiotta, “Impact of different spray-drying conditions on the viability of wine Saccharomyces cerevisiae strains,” World Journal of Microbiology and Biotechnology, vol. 32, no. 1, pp. 1–9, 2016.
[45] K. Rawat, A. Kumari, R. Kumar, P. Ahlawat, and S. C. Sindhu, “Spray-dried lassi powder: Process optimisation using RSM and physicochemical properties during storage at room and refrigerated temperature,” International Dairy Journal, vol. 131, p. 105374, 2022.
[46] Z. Nale, I. Tontul, A. Aşçi Arslan, H. Sahin Nadeem, and A. Kucukcetin, “Microbial viability, physicochemical and sensory properties of kefir microcapsules prepared using maltodextrin/Arabic gum mixes,” International Journal of Dairy Technology, vol. 71, pp. 61–72, 2018.
[47] K. Vivek, S. Mishra, and R. C. Pradhan, “Optimization of Spray drying conditions for developing nondairy based probiotic Sohiong fruit powder,” International Journal of Fruit Science, vol. 21, no. 1, pp. 193–204, 2021.
[48] P. Darvishzadeh, V. Orsat, and S. P. Faucher, “Encapsulation of Russian olive water kefir as an innovative functional drink with high antioxidant activity,” Plant Foods for Human Nutrition, vol. 76, no. 2, pp. 161–169, 2021.
[49] N. N. Alves, G. Ben Messaoud, S. Desobry, J. M. C. Costa, and S. Rodrigues, “Effect of drying technique and feed flow rate on bacterial survival and physicochemical properties of a non-dairy fermented probiotic juice powder,” Journal of Food Engineering, vol. 189, pp. 45–54, 2016.
[50] K. Thirugnanasambandham and V. Sivakumar, “Influence of process conditions on the physicochemical properties of pomegranate juice in spray drying process: Modelling and optimization,” Journal of the Saudi Society of Agricultural Sciences, vol. 16, no. 4, pp. 358–366, 2017.
[51] S. Behboudi-Jobbehdar, C. Soukoulis, L. Yonekura, and I. Fisk, “Optimization of spray-drying process conditions for the production of maximally viable microencapsulated L. acidophilus NCIMB 701748,” Drying Technology, vol. 31, no. 11, pp. 1274–1283, 2013.
[52] D. Petkova, D. Mihaylova, and I. Desseva, “Microencapsulation in food industry – An overview,” BIO Web of Conferences, vol. 45, p. 02005, 2022.
[53] S. Talebian, T. Schofield, P. Valtchev, A. Schindeler, J. M. Kavanagh, Q. Adil et al., “Biopolymer‐based multilayer microparticles for probiotic delivery to colon,” Advanced Healthcare Materials, vol. 11, no. 11, pp. 1–25, 2022.
[54] M. D. Azhar, S. A. Hashib, U. K. Ibrahim, and N. A. Rahman, “Development of carrier material for food applications in spray drying technology: An overview,” Materials Today: Proceedings, vol. 47, pp. 1371–1375, 2021.
[55] A. Pasha, S. Utekar, and R. Ghosh, “Recent advances in microencapsulation technology and their applications,” The Bombay Technologist, vol. 68, no. 1, p. 125162, 2021.
[56] J. K. M. Lee, F. S. Taip, and H. Z. Abdulla, “Effectiveness of additives in spray drying performance: A review,” Food Research, vol. 2, no. 6, pp. 486–499, 2018.
[57] A. Kocira, K. Kozłowicz, K. Panasiewicz, M. Staniak, E. Szpunar-Krok, and P. Hortyńska, “Polysaccharides as edible films and coatings: Characteristics and influence on fruit and vegetable quality—A Review,” Agronomy, vol. 11, no. 5, p. 813, 2021.
[58] Z. Xiao, J. Xia, Q. Zhao, Y. Niu, and D. Zhao, “Maltodextrin as wall material for microcapsules: A review,” Carbohydrate Polymers, vol. 298, p. 120113, 2022.
[59] M. C. Sweedman, M. J. Tizzotti, C. Schäfer, and R. G. Gilbert, “Structure and physicochemical properties of octenyl succinic anhydride modified starches: A review,” Carbohydrate Polymers, vol. 92, no. 1, pp. 905–920, 2013.
[60] S. Arslan, M. Erbas, I. Tontul, and A. Topuz, “Microencapsulation of probiotic Saccharomyces cerevisiae var: Boulardii with different wall materials by spray drying,” LWT-Food Science and Technology, vol. 63, no. 1, pp. 685–690, 2015.
[61] M. Milivojević, A. Popović, I. Pajić-Lijaković, I. Šoštarić, S. Kolašinac, and Z. D. Stevanović, “Alginate gel-based carriers for encapsulation of carotenoids: On challenges and applications,” Gels, vol. 9, no. 8, p. 620, 2023.
[62] D. Arepally and T. K. Goswami, “Effect of inlet air temperature and gum Arabic concentration on encapsulation of probiotics by spray drying,” LWT-Food Science and Technology, vol. 99, pp. 583–593, 2019.
[63] A. Xie, S. Zhao, Z. Liu, X. Yue, J. Shao, M. Li et al., “Polysaccharides, proteins, and their complex as microencapsulation carriers for delivery of probiotics: A review on carrier types and encapsulation techniques,” International Journal of Biological Macromolecules, vol. 242, p. 124784, 2023.
[64] S. Minj and S. Anand, “Development of a spray-dried conjugated whey protein hydrolysate powder with entrapped probiotics,” Journal of Dairy Science, vol. 105, no. 3, pp. 2038–2048, 2022.
[65] M. Fathi, F. Donsi, and D. J. McClements, “Protein‐based delivery systems for the nanoencapsulation of food ingredients,” Comprehensive Reviews in Food Science and Food Safety, vol. 17, no. 4, pp. 920–936, 2018.
[66] J. Hadzieva, K. Mladenovska, M. Simonoska Crcarevska, M. Glavaš Dodov, S. Dimchevska, N. Geškovski, et al., “Lactobacillus casei loaded soy protein-alginate microparticles prepared by spray-drying,” Food Technology and Biotechnology, vol. 55, no. 2, pp. 173–186, 2017.
[67] J. Jiang, C. Ma, X. Song, J. Zeng, L. Zhang, and P. Gong, “Spray drying co-encapsulation of lactic acid bacteria and lipids: A review,” Trends in Food Science & Technology, vol. 129, pp. 134–143, 2022.
[68] N. K. Mohammed, C. P. Tan, Y. A. Manap, B. J. Muhialdin, and A. S. M. Hussin, “Spray Drying for the encapsulation of oils—A Review,” Molecules, vol. 25, no. 17, p. 3873, 2020.
[69] N. Ngamekaue, T. Dumrongchai, A. Rodklongtan, and P. Chitprasert, “Improving probiotic survival through encapsulation in coconut oil in whey protein isolate emulsions during spray drying and gastrointestinal digestion,” LWT - Food Science Technology, vol. 198, p. 116061, 2024.
[70] D. Eratte, T. R. Gengenbach, K. Dowling, C. J. Barrow, and B. Adhikari, “Survival, oxidative stability, and surface characteristics of spray dried co-microcapsules containing omega-3 fatty acids and probiotic bacteria,” Drying Technology, vol. 34, no. 16, pp. 1926–1935, 2016.
[71] Q. Sun, S. Yin, Y. He, Y. Cao, and C. Jiang, “Biomaterials and encapsulation techniques for probiotics: current status and future prospects in biomedical applications,” Nanomaterials, vol. 13, no. 15, p. 2185, 2023.
[72] J. Kim, N. Muhammad, B. H. Jhun, and J.-W. Yoo, “Probiotic delivery systems: A brief overview,” Journal of Pharmaceutical Investigation, vol. 46, no. 4, pp. 377–386, 2016.
[73] E. Akanny, S. Bourgeois, A. Bonhommé, A. Doleans-Jordheim, F. Bessueille, and C. Bordes, “Development of enteric polymer-based microspheres by spray-drying for colonic delivery of Lactobacillus rhamnosus GG,” International Journal of Pharmaceutics, vol. 584, p. 119414, 2020.
[74] M. U. H. Joardder, M. H. Bosunia, M. M. Hasan, A. A. Ananno, and A. Karim, “Significance of glass transition temperature of food material in selecting drying condition: An in-depth analysis,” Food Reviews International, vol. 40, no. 3, pp. 952–973, 2024.
[75] J. Perdana, M. B. Fox, C. Siwei, R. M. Boom, and M. A. I. Schutyser, “Interactions between formulation and spray drying conditions related to survival of Lactobacillus plantarum WCFS1,” Food Research International, vol. 56, pp. 9–17, 2014.
[76] K. Samborska, S. Boostani, M. Geranpour, H. Hosseini, C. Dima, S. Khoshnoudi-Nia, “Green biopolymers from by-products as wall materials for spray drying microencapsulation of phytochemicals,” Trends in Food Science & Technology, vol. 108, pp. 297–325, 2021.
[77] M. Souza, A. Mesquita, C. Veríssimo, C. Grosso, A. Converti, and M. I. Maciel, “Microencapsulation by spray drying of a functional product with mixed juice of acerola and ciriguela fruits containing three probiotic lactobacilli,” Drying Technology, vol. 40, no. 6, pp. 1185–1195, 2022.
[78] N. Phisut, “Spray drying technique of fruit juice powder: Some factors influencing the properties of product,” Spray Dry. Tech. fruit juice powder some factors Influ. Prop. Prod., vol. 19, no. 4, pp. 1297–1306, 2012.
[79] C. O. Dias, J. S. O. de Almeida, S. S. Pinto, F. C. de Oliveira Santana, S. Verruck, C. M. O. Müller et al., “Development and physico-chemical characterization of microencapsulated bifidobacteria in passion fruit juice: A functional non-dairy product for probiotic delivery,” Food Biosci., vol. 24, pp. 26–36, 2018.
[80] N. N. Alves, G. Ben Messaoud, S. Desobry, J. M. C. Costa, and S. Rodrigues, “Effect of drying technique and feed flow rate on bacterial survival and physicochemical properties of a non-dairy fermented probiotic juice powder,” Journal of Food Engineering, vol. 189, pp. 45–54, Nov. 2016.
[81] A. Wang, J. Lin, and Q. Zhong, “Probiotic powders prepared by mixing suspension of Lactobacillus salivarius NRRL B-30514 and spray-dried lactose: Physical and microbiological properties,” Food Research International, vol. 127, p. 108706, 2020.
[82] K. Muzaffar, G. A. Nayik, and P. Kumar, “Stickiness problem associated with spray drying of sugar and acid rich foods,” Food and Nutrition Sciences, vol. S12:003, pp. 11–13, 2015.
[83] Y. Su, X. Zheng, Q. Zhao, N. Fu, H. Xiong, W. D. Wu et al., “Spray drying of Lactobacillus rhamnosus GG with calcium-containing protectant for enhanced viability,” Powder Technology, vol. 358, pp. 87–94, 2019.
[84] A. P. Cahya, M. Syaflan, and N. Ngatirah, “Probiotic (Lactobacillus casei) encapsulation using the method of spray drying with combined prebiotic from Iles-Iles (Amorphopallus oncophyllus) and protectant agent (skim milk, gum arabic, maltodextrin),” Indonesian Food and Nutrition Progress, vol. 15, no. 2, p. 61, 2018.
[85] B. C. Salazar Alzate, M. Cortés Rodríguez, and O. I. Montoya Campuzano, “The impact of storage conditions on the stability of sugarcane powder biofortified with kefir grains,” Revista Facultad Nacional de Agronomía Medellín, vol. 68, no. 2, pp. 7703–7712, 2015.
[86] M. Fazaeli, Z. Emam-Djomeh, A. Kalbasi Ashtari, and M. Omid, “Effect of spray drying conditions and feed composition on the physical properties of black mulberry juice powder,” Food and Bioproducts Processing, vol. 90, no. 4, pp. 667–675, 2012.
[87] R. Sasikumar, K. Vivek, and A. K. Jaiswal, “Effect of spray drying conditions on the physical characteristics, amino acid profile, and bioactivity of blood fruit (Haematocarpus validus Bakh.F. Ex Forman) seed protein isolate,” Journal of Food Processing and Preservation, vol. 45, no. 6, pp. 1–14, 2021.
[88] M. Cabello-Olmo, M. Oneca, P. Torre, J. Vicente Díaz, I. J. Encio, M. Barajas et al., “Influence of storage temperature and packaging on bacteria and yeast viability in a plant-based fermented food,” Foods, vol. 9, no. 3, p. 302, 2020.
[89] N. Juárez-Trujillo, M. Jiménez-Fernández, E. Franco-Robles, C. I. Beristain-Guevara, M. A. Chacón-López, and R. I. Ortiz-Basurto, “Effect of three-stage encapsulation on survival of emulsified Bifidobacterium animalis subsp. Lactis during processing, storage and simulated gastrointestinal tests,” LWT - Food Science Technology, vol. 137, p. 110468, 2021.
[90] E. Martins, D. C. Cnossen, C. R. J. Silva, J. C. C. Junior, L. A. Nero, I. T. Perrone et al., “Determination of ideal water activity and powder temperature after spray drying to reduce Lactococcus lactis cell viability loss,” Journal of Dairy Science, vol. 102, no. 7, pp. 6013–6022, 2019.
[91] D. Dianawati, V. Mishra, and N. P. Shah, “Survival of microencapsulated probiotic bacteria after processing and during storage: A Review,” Crit. Rev. Food Sci. Nutr., vol. 56, no. 10, pp. 1685–1716, 2016.
[92] M. Mizielińska et al., “The influence of immobilization and packaging on the viability of probiotics stored at 25°C,” World Scientific News, vol. 77, no. 2, pp. 124–143, 2017, [Online]. Available: www.worldscientificnews.com
[93] C. S. Ranadheera, J. K. Vidanarachchi, R. S. Rocha, A. G. Cruz, and S. Ajlouni, “Probiotic delivery through fermentation: Dairy vs. non-dairy beverages,” Fermentation, vol. 3, no. 4, pp. 1–17, 2017.
[94] S. Vesterlund, K. Salminen, and S. Salminen, “Water activity in dry foods containing live probiotic bacteria should be carefully considered: A case study with Lactobacillus rhamnosus GG in flaxseed,” International Journal of Food Microbiology, vol. 157, no. 2, pp. 319–321, 2012.
[95] D. Dianawati, V. Mishra, and N. P. Shah, “Viability, acid and bile tolerance of spray dried probiotic bacteria and some commercial probiotic supplement products kept at room temperature,” Journal of Food Science, vol. 81, no. 6, pp. M1472–M1479, 2016.
[96] S. Huang, “Spray drying of probiotic bacteria: From molecular mechanism to pilot-scale production,” Ph.D dissertation, School of Chemical and Environmental Engineering, Soochow University, China, 2020. [Online]. Available: https://hal.science/tel-02791240/
[97] A. Terpou, A. Papadaki, I. Lappa, V. Kachrimanidou, L. Bosnea, and N. Kopsahelis, “Probiotics in food systems: significance and emerging strategies towards improved viability and delivery of enhanced beneficial value,” Nutrients, vol. 11, no. 7, p. 1591, 2019.
[98] P. Chaikham, P. Rattanasena, and P. Chunthanom, “Survival of Lactobacillus casei 01 in probiotic-supplemented Mamao (Antidesma bunius) juice powder during storage,” International Food Research Journal, vol. 25, no. 5, pp. 2149–2156, 2018.
[99] G. L. Nunes, M. H. Motta, A. J. Cichoski, R. Wagner, É. I. Muller, C. F. Codevilla et al., “Encapsulation of Lactobacillus acidophilus La-5 and Bifidobacterium Bb-12 by spray drying and evaluation of its resistance in simulated gastrointestinal conditions, thermal treatments and storage conditions,” Ciência Rural, vol. 48, no. 6, pp. 1–11, 2018.
[100] T. Zhang, C. Shang, T. Du, J. Zhuo, C. Wang, B. Li et al., “Cytoprotection of probiotics by nanoencapsulation for advanced functions,” Trends in Food Science & Technology, vol. 142, p. 104227, 2023.
[101] P. Singh, B. Medronho, L. Alves, G. J. da Silva, M. G. Miguel, and B. Lindman, “Development of carboxymethyl cellulose-chitosan hybrid micro- and macroparticles for encapsulation of probiotic bacteria,” Carbohydrate Polymers, vol. 175, pp. 87–95, 2017.
[102] A. Y. Bustos, G. F. de Valdez, R. Raya, A. M. de Almeida, S. Fadda, and M. P. Taranto, “Proteomic analysis of the probiotic Lactobacillus reuteri CRL1098 reveals novel tolerance biomarkers to bile acid-induced stress,” Food Research International, vol. 77, pp. 599–607, 2015.
[103] L. Liu, S. E. Helal, and N. Peng, “CRISPR-Cas-based engineering of probiotics,” BioDesign Research, vol. 5, pp. 1–12, 2023.
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