Bio-cementation of tropical lateritic soil via microbially induced calcite precipitation: Strength enhancement and microstructural mechanisms
DOI:
https://doi.org/10.15282/construction.v6i1.13737Keywords:
Biochemical, Morphology, Laterite, Bacillus subtilis, Unconfined compressive strength, MICPAbstract
This study investigates the effectiveness of Microbially Induced Calcite Precipitation (MICP) using Bacillus subtilis as a sustainable technique for biochemically stabilizing laterite soil. Lateritic soils, common in tropical regions, are problematic in construction due to high porosity, low strength, and brittle stress–strain responses. The main objectives were to characterize the physical properties of untreated laterite soil, evaluate the effect of MICP treatment on its stress–strain behaviour, and correlate mechanical improvements with microstructural changes using scanning electron microscopy (SEM) and quantitative image analysis. Results showed that untreated soil exhibited low peak stress (52 kN/m2) and brittle failure dominated by splitting cracks. Following MICP treatment, peak stresses improved markedly to 235 kN/m2 at 7 days, 296 kN/m2 at 14 days, and 420 kN/m2 at 30 days of curing. This shows that the strength exceeds commonly reported minimum requirements for stabilized subgrades in tropical pavement systems. Stress–strain curves showed higher stiffness, improved ductility, and more stable post-peak behaviour, while failure shifted toward cleaner shear cracking. The morphological analysis by SEM and image analyses confirmed progressive calcite precipitation, void filling, and stronger particle bonding over curing time. This study advances current knowledge by systematically integrating mechanical testing with microstructural quantification to elucidate the mechanics governing bio-cementation in laterite soils. These findings establish MICP as a low-carbon, environmentally friendly alternative to conventional chemical stabilizers, offering promising applications for subgrades and foundations in tropical geotechnical engineering.
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References
[1] M. Aguiar, "Tropical soils: considerations on occurrence and characteristics and studies in Brazil," New Approaches in Foundation Engineering, London: IntechOpen, 2022. https://doi.org/10.5772/intechopen.103947
[2] A.M.A. Ezreig, M.A.M. Ismail, K.I.A. Ehwailat, "A state of review: challenges and techniques of laterite soil stabilisation using chemical, economical, and eco-friendly materials," Innovative Infrastructure Solutions, vol. 7, no. 3, p. 229, 2022. https://doi.org/10.1007/s41062-022-00821-z
[3] S. Tamassoki, N. Norsyahariati, F. Mohd Jakarni, F. Mohd Kusin, A.S.A. Rashid, and M.J. Roshan, "Compressive and shear strengths of coir fibre reinforced activated carbon stabilised lateritic soil," Sustainability, vol. 14, no. 15, p. 9100, 2022. https://doi.org/10.3390/su14159100
[4] M. Zhao, J. Gu, M.H. El Naggar, H. Liu, G. Liu, and T. Wang, "Intrinsic compressibility of laterites and lateritic soils," Canadian Geotechnical Journal, vol. 61, no. 12, pp. 2774–2784, 2024. https://doi.org/10.1139/cgj-2023-0610
[5] V.O. Kühn, B.C.F.L. Lopes, C.R. Borges, N.S. Massocco, and M.P. Cordão-Neto, "Relationship between pore-size distribution and compressibility of a lateritic soil," Géotechnique Letters, vol. 11, no. 4, pp. 326–332, 2021. https://doi.org/10.1680/jgele.21.00063
[6] I.A. Okewale, and H. Grobler, "Role of fabrics and compositions on the geomechanical characteristics of lateritic soils," Indian Geotechnical Journal, vol. 56, pp. 2831–2844, 2025. https://doi.org/10.1007/s40098-025-01318-z
[7] T. Waikhamnuan, S. Chawchai, and R. Bissen, "Geochemistry of laterite around UNESCO World Heritage Sites in Kamphaeng Phet, Thailand," Heritage Science, vol. 13, no. 1, p. 17, 2025. https://doi.org/10.1038/s40494-025-01645-7
[8] N. Daud, and M. Rusdi, "Fly ash stabilized lateritic soil as subbase material: A review," Earth Science Malaysia, vol. 6, no. 1, pp. 15–23, 2022. https://doi.org/10.26480/esmy.01.2022.15.23
[9] N.A. Wahab, A.S.A. Rashid, M.J. Roshan, et al., "Effects of cement on the compaction properties of lateritic soil," in IOP Conference Series: Materials Science and Engineering, vol. 1153, 2021, p. 012015. https://doi.org/10.1088/1757-899X/1153/1/012015
[10] Y. Izumi, A. Iizuka, and H.J. Ho, "Calculation of greenhouse gas emissions for a carbon recycling system using mineral carbon capture and utilization technology in the cement industry," Journal of Cleaner Production, vol. 312, p. 127618, 2021. https://doi.org/10.1016/j.jclepro.2021.127618
[11] M.A. Jaber, N. Muhammad, and H.F. Ahmad, "Microbial screening and morphological analysis of the peat soil gebeng, malaysia," in Journal of Physics: Conference Series, vol. 3003, 2025, p. 012026. https://doi.org/10.1088/1742-6596/3003/1/012026
[12] M.A. Jaber, N. Muhammad, and A. Namdar, "Influence of by-product gypsum and concrete waste on the structural and mineralogical properties of peat soil," in IOP Conference Series: Earth Environmental Science, vol. 1509, 2025, p. 012007. https://doi.org/10.1088/1755-1315/1509/1/012007
[13] F.O. Okafor, and E.A. Egbe, "Potentials of cement kiln dust in sub-grade improvement," Nigerian Journal of Technology, vol. 32, no. 1, pp. 109–116, 2013. https://doi.org/10.4314/njt.321.614
[14] A.A. Amadi, and A.O. Eberemu, "Potential application of lateritic soil stabilized with cement kiln dust (ckd) as liner in waste containment structures," Geotechnical and Geological Engineering, vol. 31, no. 4, pp. 1221–1230, 2013. https://doi.org/10.1007/s10706-013-9645-3
[15] A. Almsedeen, N. Muhammad, and M. Ishak, "Preliminary strength characterization of peat soil stabilized with Mg-rich gypsum waste" in AIP Conf. Proc., vol. 3014, no 1, 2024, p. 030003. https://doi.org/10.1063/5.0196830
[16] A. Almsedeen, N. Muhammad, and M. Ishak, "Waste to product: potential of mg-rich gypsum additive for improvement of peat soil," in Proc. of the Green Materials and Electronic Packaging Interconnect Technology Symposium, 2023, pp. 263–270. https://doi.org/10.1007/978-981-19-9267-4_29
[17] A. Almsedeen, and M. Fakhrurrazi, N. Muhammad, S. Siddiqua, M.N. Zakaria, "Rejuvenating organic soil behavior with crushed waste concrete: experimental and mineralogical investigations," International Journal of Integrated Engineering, vol. 16, no. 9, pp. 130–142, 2024. https://doi.org/10.30880/ijie.2024.16.09.010
[18] A.A. Busari, I.I. Akinwumi, P.O. Awoyera, O.M. Olofinnade, T.I. Tenebe, and J.C. Nwanchukwu, "Stabilization effect of aluminum dross on tropical lateritic soil," International Journal of Engineering Research in Africa, vol. 39, pp. 86–96, 2018. https://doi.org/10.4028/www.scientific.net/JERA.39.86
[19] P. Padalkar, T. Kulkarni, and C. Joshi, "Study on properties of lateritic soil using fly ash and coir fibers," International Journal of Engineering Research in Mechanical and Civil Engineering, vol. 6, no. 3, pp. 428–436, 2017.
[20] E.P.K. Onyelowe, D.B. Van, C. Ezugwu, T. Amhadi, F. Sosa, and W. Wu, "Experimental assessment of subgrade stiffness of lateritic soils treated with crushed waste plastics and ceramics for pavement foundation," Int. J. of Low-Carbon Technol., vol. 14, no.1, pp. 1–18, Mar. 2019. https://doi.org/10.1093/ijlct/ctz015
[21] B.A. Akshatha, A. Jain, and Z. Ahmed, "Stabilization of laterite soil with waste tire rubber," International Journal of Engineering Sciences & Research Technology, vol. 7, no. 12, pp. 181–187, 2018.
[22] J. Menon, and M.S. Ravikumar, "Strength evaluation of laterite soil stabilized using polymer fibers," International Journal of Civil Engineering and Technology, vol. 9, no.2, pp. 227–234, 2018.
[23] A.B. Haider, A. Iravanian, M.H. Selman, and A. Ekinci, "Using waste PET shreds for soil stabilization: efficiency and durability assessment," International Journal of Geosynthetics and Ground Engineering, vol. 9, no. 4, p. 48, 2023. https://doi.org/10.1007/s40891-023-00473-8
[24] R.R. Srivastava, D. Rajak, S. Ilyas, H. Kim, and P. Pathak, "Challenges, regulations, and case studies on sustainable management of industrial waste," Minerals, vol. 13, no. 1, p. 51, 2022. https://doi.org/10.3390/min13010051
[25] D. Barman, and S.K. Dash, "Stabilization of expansive soils using chemical additives: a review," Journal of Rock Mechanics and Geotechnical Engineering, vol. 14, no. 4, pp.1319–1342, 2022. https://doi.org/10.1016/j.jrmge.2022.02.011
[26] N. Prajapati, A. Agnihotri, and N. Basak, "Microbial induced calcite precipitation (MICP) a sustainable technique for stabilization of soil: A review," Materials Today: Proceedings, vol. 93, pp. 357–361, 2023. https://doi.org/10.1016/j.matpr.2023.07.303
[27] A. Almajed, M. Lateef, A. Moghal, and K. Lemboye, "State-of-the-art review of the applicability and challenges of microbial-induced calcite precipitation (MICP) and enzyme-induced calcite precipitation (EICP) Techniques for Geotechnical and Geoenvironmental Applications," Crystals, vol. 11, no. 4, p. 370, 2021. https://doi.org/10.3390/cryst11040370
[28] K.A. Gebru, T.G. Kidanemariam, and H.K. Gebretinsae, "Bio-cement production using microbially induced calcite precipitation (MICP) method: A review," Chemical Engineering Science, vol. 238, p. 116610, 2021. https://doi.org/10.1016/j.ces.2021.116610
[29] D. Mori, and U. Kala, "A review on qualitative interaction among the parameters affecting ureolytic microbial-induced calcite precipitation," Environmental Earth Sciences, vol. 80, no. 10, p. 329, 2021. https://doi.org/10.1007/s12665-021-09613-7
[30] V. Pacheco, L. Bragagnolo, and C. Reginatto, A. Thomé, "Microbially Induced Calcite Precipitation (MICP): review from an engineering perspective," Geotechnical and Geological Engineering, vol. 40, no. 1, pp. 2379–2396, 2022. https://doi.org/10.1007/s10706-021-02041-1
[31] S. Fazelikia, S. Abtahi, M. Kargar, and M. Jafarinia, "Microbial Induced Calcite Precipitation (MICP) potential of ureolytic bacillus sp. isolated from the soil of eroded ecosystems for stabilizing and improving the fertility of eroded soils," Geomicrobiology Journal, vol. 40, no.6, pp. 569–581, 2023. https://doi.org/10.1080/01490451.2023.2211077
[32] K. Zhang, C. Tang, N.J. Jiang, X.H. Pan, B. Liu, and Y. J. Wang, "Microbial induced carbonate precipitation (MICP) technology: a review on the fundamentals and engineering applications," Environment Earth Science, vol. 82, no. 9, p. 229, 2023. https://doi.org/10.1007/s12665-023-10899-y
[33] K.J. Osinubi, A.O. Eberemu, E.W. Gadzama, and T.S. Ijimdiya, "Plasticity characteristics of lateritic soil treated with Sporosarcina pasteurii in microbial-induced calcite precipitation application," SN Appl. Sci., vol. 1, p. 829, Jul. 2019. https://doi.org/10.1007/s42452-019-0868-7
[34] J. Sani, M. George, F. Oriola, and M. Shittu, "Effects of curing time and compactive effort on unconfined compressive strength test of microbial induced calcite precipitate-treated lateritic soil," International Journal for Modern Trends in Science and Technology, vol. 5, no. 3, pp. 642–648, Dec. 2020.
[35] K.J. Osinubi, A.O. Eberemu, S.T. Ijimdiya, and A.M. Ashiru, "Evaluation of strength properties of bacillus pumilus treated lateritic soil in microbial-induced calcite precipitation application," Journal of Biodiversity and Environmental Research, vol. 6, no.4, Nov. 2024.
[36] A. Smith, M. Pritchard, A. Edmondson, and S. Bashir, "The reduction of the permeability of a lateritic soil through the application of microbially induced calcite precipitation," Natural Resources, vol. 08, no. 05, pp. 337–352, 2017. https://doi.org/10.4236/nr.2017.85021
[37] R.K. Etim, A.O. Eberemu, T.S. Ijimdiya, and K.J. Osinubi, "Coupled effect of cementation solution, curing period, molding water content, and compactive effort on strength performance of biotreated lateritic soil for municipal solid waste containment application," Journal of Hazardous, Toxic, and Radioactive Waste, vol. 27, no. 3, p. 04023009, 2023. https://doi.org/10.1061/JHTRBP.HZENG-120
[38] B. Zhou, R. Jia, X. Chen, L. Yang, M. Duan, and F. Xiao, "Impact of bacteria-nitrogen coupling on cotton growth and nitrogen utilization under different salt stress," Agricultural Water Management, vol. 280, p. 108221, Feb. 2023. https://doi.org/10.1016/j.agwat.2023.108221
[39] A.L. McLoon, S.B. Guttenplan, D.B. Kearns, K. Roberto, and L. Richard, "Tracing the domestication of a biofilm-forming bacterium," Journal of Bacteriology, vol. 193, no. 8, pp. 2027–2034, 2011. https://doi.org/10.1128/jb.01542-10
[40] C.P.D. Quadros, M.C.T. Duarte, and G.M. Pastore, "Biological activities of a mixture of biosurfactant from bacillus subtilis and alkaline lipase from fusarium oxysporum," Brazilian Journal of Microbiology, vol. 42, no. 1, pp. 354–361, Mar. 2011. https://doi.org/10.1590/S1517-83822011000100045
[41] I.O. Fatoyinbo, S. Malomo, Y.A. Asiwaju-Bello, and A.A. Bello, "Effect of multiple-compaction on laterite soil: geotechnical and geo-statistical analysis," Scientific Reports, vol. 14, no. 1, p. 4067, 2024. https://doi.org/10.1038/s41598-024-54644-2
[42] G. Santha Kumar, P.K. Saini, R. Deoliya, A.K. Mishra, and S.K. Negi, "Characterization of laterite soil and its use in construction applications: A review," Resources, Conservation & Recycling Advances, vol. 16, p. 200120, 2022. https://doi.org/10.1016/j.rcradv.2022.200120
[43] R.K. Etim, K.J. Osinubi, A.O. Eberemu, and T.S. Ijimdiya, "Performance evaluation of compacted lateritic soil bio-cemented with bacillus megaterium for use as liners and covers in municipal solid waste containment," in Proc. Geo-Congress 2024, Vancouver, BC, Canada, 2024, pp. 176–185. https://doi.org/10.1061/9780784485330.019
[44] K. Kumar, J. Naskar, and A.K. Sharma, "Effectiveness of microbial induced calcite precipitation on sand-clay mixtures," in Proc. Geo-Congress 2023, Los Angeles, CA, USA, 2023, pp. 340–350. https://doi.org/10.1061/9780784484661.036
[45] N. Abdul Wahab, A.S.A. Rashid, M.J. Roshan, S. Horpibulsuk, and R. Razali, "Climate change impact on unsaturated cement-stabilized laterite soil using suction-controlled testing," Geotechnical and Geological Engineering, vol. 43, no. 1, pp. 585–603, 2025. https://doi.org/10.1007/s10706-024-02980-5
[46] A.K. Arshad, E. Shaffie, F. Ismail, W. Hashim, and Z.A. Rahman, "Cement stabilised soil subgrade: design and construction," International Journal of Civil Engineering and Technology, vol. 9, no. 7, pp. 1192–1200, 2018.
[47] A.I. Omoregie, E.A. Palombo, and P.M. Nissom, "Bioprecipitation of calcium carbonate mediated by ureolysis: A review," Environmental Engineering Research, vol. 26, no. 6, p. 200379, Nov. 2020. https://doi.org/10.4491/eer.2020.379
[48] C.M. Kirkland, A. Akyel, R. Hiebert, J. McCloskey, J. Kirsey, and A.B. Cunningham, "Ureolysis-induced calcium carbonate precipitation (UICP) in the presence of 1 CO2-affected brine: a field demonstration," International Journal of Greenhouse Gas Control, vol. 109, p. 103391, 2021. https://doi.org/10.1016/j.ijggc.2021.103391
[49] M. Hemayati, A. Nematollahi, E. Nikooee, G. Habibagahi, and A. Niazi, "Non‐ureolytic microbially induced carbonate precipitation: Investigating a cleaner biogeotechnical engineering pathway for soil mechanical improvement," The Journal of Engineering, vol. 2024, no. 1, p. e12350, Jan. 2024. https://doi.org/10.1049/tje2.12350
[50] Y. Wang, M. Li, H. Peng, J. Kang, H. Guo, and Y. Luo, "Improvement of unconfined compressive strength in granite residual soil by indigenous microorganisms," Sustainability, vol. 17, no. 15, p. 6895, 2025. https://doi.org/10.3390/su17156895
[51] N. Tiwari, and N. Satyam, "Indigenous bacteria-driven eco-friendly soil stabilization: a bioinspired approach through microbially induced calcite precipitation for sustainable infrastructure development," Indian Geotechnical Journal, vol. 54, no. 6, pp. 2286–2302, 2024. https://doi.org/10.1007/s40098-023-00864-8
[52] M. Sharma, and N. Satyam, "Factors affecting biomineralization and various applications of nature-based solutions," in Sustainable Materials in Civil Infrastructure, Elsevier, 2024, pp. 161–171. https://doi.org/10.1016/B978-0-443-16142-1.00009-4
[53] S. Rababah, A. Alawneh, B.A. Albiss, H.H. Aldeeky, E.J. Bani Ismaeel, and S. Mutlaq, "Enhancing soil stability through innovative microbial-induced calcium carbonate techniques with sustainable ingredient," Civil Engineering Journal, vol. 10, no. 8, pp. 2536–2553, 2024. https://doi.org/10.28991/CEJ-2024-010-08-08
[54] M. Aziz, "Mechanical properties of a high plasticity clay mixed with sand and low-plastic silt," Materials Today: Proceedings, vol. 80, pp. 1205–1211, 2023. https://doi.org/10.1016/j.matpr.2023.08.012
[55] M.F. Alarcón-Jiménez, J.H. Camacho-Tamayo, and J.H. Bernal, "Friability and its relationship with clay and organic carbon in soils cultivated with sugar cane," Agronomia Colombiana, vol. 33, no. 3, pp. 373–382, 2015.
[56] N. Kumari, and C. Mohan, "Basics of clay minerals and their characteristic properties," in Clay and Clay Minerals, IntechOpen, 2021. https://doi.org/10.5772/intechopen.97672
[57] C.K. Dzaklo, "Application of natural and/or stabilized black soils as sub-structural fills for railway foundations," Ph.D. dissertation, School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, Australia, 2023.
[58] K.A. Saeed, K.A. Kassim, H. Nur, and N.Z.M. Yunus, "Strength of lime-cement stabilized tropical lateritic clay contaminated by heavy metals," KSCE Journal of Civil Engineering, vol. 19, no. 4, pp. 887–892, 2015. https://doi.org/10.1007/s12205-013-0086-6
[59] G. Yunusa, A. Kassim, and N. Gofar, "Effect of surface flux boundary conditions on transient suction distribution in homogeneous slope," Indian Journal of Science and Technology, vol. 7, no.12, pp. 2064–2075, 2014. https://doi.org/10.17485/ijst/2014/v7i12.23
[60] M. Uba Zango, K.A. Kassim, R. Sa’ari, M.F.A. Rashid, A.S. Muhammed, and K. Ahmad, "Use of digital image technique to study leachate penetration in bio cemented residual soil," Materials Today: Proceedings, vol. 48, pp. 734–740, 2021. https://doi.org/10.1016/j.matpr.2021.02.211
[61] P. Sudla, J. Donrak, M. Hoy, S. Horpibulsuk, A. Arulrajah, and A.S.A. Rashid, "Laboratory investigation of cement-stabilized marginal lateritic soil by crushed slag–fly ash replacement for pavement applications," Journal of Materials in Civil Engineering, vol. 32, no. 2, pp. 04019353, 2020. https://doi.org/10.1061/(ASCE)MT.1943-5533.0003011
[62] B. Lekha, G. Sarang, and A.R. Shankar, "Evaluation of lateritic soil stabilized with arecanut coir for low volume pavements," Transportation Geotechnics, vol. 2, pp. 20–29, 2014. https://doi.org/10.1016/j.trgeo.2014.09.001
[63] Q. Mu, L. Meng, Y. Shen, C. Zhou, and Z. Gu, "Effects of clay content on the desiccation cracking behavior of low-plasticity soils," Bulletin of Engineering Geology and the Environment, vol. 82, no. 8, p. 317, 2023. https://doi.org/10.1007/s10064-023-03346-6
[64] M. Silveira, P. Lodi, N. Correia, R. Rodrigues, and H. Giacheti, "Effect of recycled polyethylene terephthalate strips on the mechanical properties of cement-treated lateritic sandy soil," Sustainability, vol. 12, no. 23, p. 9801, 2020. https://doi.org/10.3390/su12239801
[65] A. Eisazadeh, K. Kassim, and H. Nur, "Characterization of phosphoric acid- and lime-stabilized tropical lateritic clay," Environmental Earth Sciences, vol. 63, no.4, pp. 1057–1066, 2011. https://doi.org/10.1007/s12665-010-0781-2
[66] A.S.A Rashid, S. Tabatabaei, N.Z.M. Yunus, and W.W. Hassan, "Shear strength improvement of lateritic soil stabilized by biopolymer based stabilizer," Geotechnical and Geological Engineering, vol. 37, no.6, pp. 5533–5541, 2019. https://doi.org/10.1007/s10706-019-00944-8
[67] Y.B. Yamus, A.S.A. Rashid, K. Ahmad, and N.A. Rahman, "A review on the fundamental engineering properties of compacted laterite soil at different gradations," Journal of Advanced Research in Applied Sciences and Engineering Technology, vol. 16, no. 1, pp. 10–19, 2019. https://www.akademiabaru.com/doc/ARASETV16_N1_P10_19.pdf
[68] T. Woldesenbet, T. Petros, Z. Rabba, and E. Quezon, "Developing a numerical models to predict moisture-density relationship from the index properties of lateritic soils," Indian Geotechnical Journal, vol. 55, no. 2, pp. 827–844, Apr. 2025. https://doi.org/10.1007/s40098-024-00944-3
[69] M. Arabani, M.M. Shalchian, and A. Baghbani, "A state-of-the-art review on interactive mechanisms and multi-scale application of biopolymers (BPs) in geo-improvement and vegetation growth," Journal of Environmental Management, vol. 358, p. 120905, 2024. https://doi.org/10.1016/j.jenvman.2024.120905
[70] G. Li, Y. Zhang, X. Hua, J. Liu, and X. Liu, "Mechanical properties of aeolian sand cemented via microbially induced calcite precipitation (MICP)," Scientific Reports, vol. 14, no. 1, p. 22745, 2024. https://doi.org/10.1038/s41598-024-73986-5
[71] J. Liu, Y. Meng, Y. Zhang, X. Ji, Z. Zheng, and L. Wang, "Effect of phosphoric acid on the properties of sodium bentonite and its mechanism," Molecules, vol. 30, p. 843, 2025. https://doi.org/10.3390/molecules30040843
[72] L. Afolagboye, A. Talabi, O. Owoyemi, "The use of Polidori’s plasticity and activity charts in classifying some residual lateritic soils from Nigeria," Heliyon, vol. 7, no.8, pp. e07713, 2021. https://doi.org/10.1016/j.heliyon.2021.e07713
[73] U. Rochanavibhata, S. Lhajai, P. Jongpradist, N. Makaratat, and P. Jamsawang, "Enhancing mechanical and microstructural properties of lateritic soil using bottom ash and cement stabilization: a comparative analysis of destructive and nondestructive testing methods," Cogent Engineering, vol. 12, no. 1, p. 2524443, 2025. https://doi.org/10.1080/23311916.2025.2524443
[74] N. Thanasisathit, T. Chompoorat, P. Jongpradist, W. Kitisatorn, and P. Jamsawang, "Toughness enhancement of geopolymer stabilized laterites using para rubber latex for sustainable base and subbase applications," Scientific Reports, vol. 16, no. 1, p. 2147, 2026. https://doi.org/10.1038/s41598-025-31957-4
[75] L.S. Tang, and Y.X. Wang, "Study on the internal mechanics and energy characteristics of soil under different failure modes," Applied Sciences, vol. 13, no. 15, p. 8648, 2023. https://doi.org/10.3390/app13158648
[76] M.A. Adajar, J. Tan, A. Adriano, S. Vera, J. Manabat, and H. Navarro, "Agar biopolymer as a sustainable alternative binder to enhance the strength of low-plasticity soil," Polymers, vol. 17, no. 9, p. 1253, 2025. https://doi.org/10.3390/polym17091253
[77] M. Zumrawi, "Construction problems of light structures founded on ESs in Sudan," International Journal of Science and Research, vol. 4, no. 8, pp. 896–902, 2015. https://doi.org/10.13140/RG.2.2.10963.86562
[78] A. Ali, N. Zhang, and R. Santos, "Mineral characterization using scanning electron microscopy (SEM): a review of the fundamentals, advancements, and research directions," Applied Sciences, vol. 13, no. 23, p. 12600, 2023. https://doi.org/10.3390/app132312600
[79] R.L. Skuce, D.J. Tobler, I. MacLaren, M.R. Lee, V.R. Phoenix, "Immobilization of nanoparticles by occlusion into microbial calcite," Chemical Geology, vol. 453, pp. 72–79, 2017. https://doi.org/10.1016/j.chemgeo.2017.02.005
[80] J. Yin, W. Qu, Z. Yibulayimu, J. Qu, "Enhancing aeolian sand stability using microbially induced calcite precipitation technology," Scientific Reports, vol. 14, no. 1, p. 23876, 2024. https://doi.org/10.1038/s41598-024-74170-5
[81] J.T. DeJong, B.M. Mortensen, B.C. Martinez, D.C. Nelson, "Bio-mediated soil improvement," Ecological Engineering, vol. 36, no. 2, pp. 197–210, 2010. https://doi.org/10.1016/j.ecoleng.2008.12.029
[82] N. Soon, L. Lee, C. Tan, H. Siew Ling, "Improvements in engineering properties of soils through microbial-induced calcite precipitation," KSCE Journal of Civil Engineering, vol. 17, no. 4, pp. 718–728, 2013. https://doi.org/10.1007/s12205-013-0149-8
[83] C. Qian, X. Ren, Y. Rui, K. Wang, "Characteristics of bio-CaCO3 from microbial bio-mineralization with different bacteria species," Biochemical Engineering Journal, vol. 176, p. 108180, 2021. https://doi.org/10.1016/j.bej.2021.108180
[84] Y.S. Jhuo, H.E. Wong, H.H. Tung, L. Ge, "Effectiveness of microbial induced carbonate precipitation treatment strategies for sand," Environmental Technology & Innovation, vol. 38, p. 104132, 2025. https://doi.org/10.1016/j.eti.2025.104132
[85] T. Fu, A.C. Saracho, S.K. Haigh, "Microbially induced carbonate precipitation (MICP) for soil strengthening: A comprehensive review," Biogeotechnics, vol. 1, no. 1, p. 100002, 2023. https://doi.org/10.1016/j.bgtech.2023.100002
[86] M. Umar, K.A. Kassim, M.U. Zango, A.S. Muhammed, "Performance evaluation of lime and microbial cementation in residual soil improvement," in IOP Conf. Series: Mater. Sci. Eng., vol. 527, 2019, p. 012005. https://doi.org/10.1088/1757-899X/527/1/012005
[87] A.I. Omoregie, K. Muda, D.E.L. Ong, O.O. Ojuri, M.K.B. Bakri, and M.M. Rahman, "Soil bio-cementation treatment strategies: state-of-the-art review," Geotechnical Research, vol. 11, no. 1, pp. 3–27, 2023. https://doi.org/10.1680/jgere.22.00051
[88] M.M. Rahman, R. Hora, I. Ahenkorah, S. Beecham, M. Karim, A. Iqbal, "State-of-the-art review of microbial-induced calcite precipitation and its sustainability in engineering applications," Sustainability, vol. 12, no. 15, p. 6281, 2020. https://doi.org/10.3390/su12156281
[89] N. Erdmann, S. Schaefer, T. Simon, A. Becker, U. Bröckel, D. Strieth, "MICP treated sand: insights into the impact of particle size on mechanical parameters and pore network after biocementation," Discover Materials, vol. 4, no. 1, p. 45, 2024. https://doi.org/10.1007/s43939-024-00108-3
[90] M.A. Jaber, N. Muhammad, H.F. Ahmad, "Sustainable stabilization using gypsum and concrete waste enhanced microbially induced calcium precipitation (MICP) activities in peat soil," Biogeotechnics, vol. 4, p. 100233, 2026. https://doi.org/10.1016/j.bgtech.2026.100233
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